UDP-glucose: Glucan Synthetase in Developing Cotton Fibers
نویسندگان
چکیده
منابع مشابه
UDP-glucose: Glucan Synthetase in Developing Cotton Fibers: II. Structure of the Reaction Product.
The solubility properties, composition, and structure of the radioactive product synthesized from UDP-[(14)C]glucose by a highly active cotton fiber glucan synthetase have been determined. Product obtained under the following three different conditions was analyzed: at high and low substrate concentrations by detached fibers, and at high substrate concentrations with an isolated particulate pre...
متن کاملEffect of Boron on the Incorporation of Glucose from UDP-Glucose into Cotton Fibers Grown in Vitro.
Boron is required for fiber growth and development in cotton ovules cultured in vitro. Incorporation of [(14)C]glucose by such fiber from supplied UDP-[(14)C]glucose into the hot alkali-insoluble fraction is rapid and linear for about 30 minutes. Incorporation of [(14)C]glucose from such substrate by fibers grown in boron-deficient ovule cultures is much less than in the case with fibers from o...
متن کاملCharacterization of Developing Cotton Fibers by Confocal Raman Microscopy
Cellulose deposition in developing cotton fibers has been studied previously with analytical techniques, such as Fourier transform infrared spectroscopy (FTIR), High-performance liquid chromatography (HPLC) and Thermogravimetric analysis (TGA). Recent technological developments in instrumentation have made Raman microscopy emerge as an extraordinary analytical tool in biological and plant resea...
متن کاملSusceptibility of UDP-Glucose:(1,3)-beta-Glucan Synthase to Inactivation by Phospholipases and Trypsin.
UDP-glucose:(1,3)-beta-glucan synthase from Beta vulgaris L. was rapidly inactivated by treatment with phospholipases C, D, and A(2). Enzyme activity could not be restored to the phospholipase-treated enzyme by the addition of phosphatidylethanolamine or other phospholipids. Membrane-bound and solubilized glucan synthase were also trypsin-labile with inactivation rates equal in the presence or ...
متن کاملThe dual functions of WLIM1a in cell elongation and secondary wall formation in developing cotton fibers.
LIN-11, Isl1 and MEC-3 (LIM)-domain proteins play pivotal roles in a variety of cellular processes in animals, but plant LIM functions remain largely unexplored. Here, we demonstrate dual roles of the WLIM1a gene in fiber development in upland cotton (Gossypium hirsutum). WLIM1a is preferentially expressed during the elongation and secondary wall synthesis stages in developing fibers. Overexpre...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1977
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.59.4.719